From panel design to validation, give your assay a fit for purpose approach with regular conversation throughout your project
Panel Design
● Ab selection (clone) ● Fluorochrome selection
Feasibility
● With the same matrix as for the clinical trial ● Test the staining protocol instrument setup ● Compensation ● And more
Gating
● Identify the populations of interest ● Assess the need for controls (FMO or Isotype) ● Define gating sequence for final reporting
Validation
● Inter/Intra assay precision ● Inter-operator and inter-instrument ● Sample stability ● Additional parameters (e.g post stained stability, sensitivity (LLOQ))
Custom Flow Cytometry Assays
With a customized flow cytometry assay development and validation approach, Cerba Research utilizes the appropriate sample types for your project.
![A scientist standing in a lab whilst testing samples](https://staging.cerbaresearch.com/app/uploads/2022/07/cerba-ghent_20-scaled.jpg)
![a scientist in a lab operating a BD FACASDuet sample preparation system](https://staging.cerbaresearch.com/app/uploads/2022/07/Cerba-US-Expansion-Tour-VF.00_00_13_14.Still001.jpg)
The Importance of Validation
All biomarkers must undergo advanced validation to ensure data is interpretable within multiple time points, within large patient cohorts, and tested at different locations around the world.
All the markers used during your study undergo advanced validation to guarantee the interpretation of data
Flow Cytometry Assay Specimens
Cerba Research is able to perform your flow cytometry assay with whole blood, bone marrow aspirate or PBMCs.
![In this video, Cerba Research takes you on an in-depth tour of their CerbACT immuno-oncology center of excellence in Asia.](https://staging.cerbaresearch.com/app/uploads/2022/07/cerba-ghent_8-scaled.jpg)
![5 scientists standing in a circle smiling and looking down at the camera. They have all put their hands together in a huddle](https://staging.cerbaresearch.com/app/uploads/2022/07/joined-hands.png)
Harmonized
Our custom flow cytometry assays are harmonized with global SOPs and standardized instrument settings for immunophenotyping and fluorescence quantification.
![Cerba US Expansion Tour VF.00_04_27_19.Still010](https://staging.cerbaresearch.com/app/uploads/2022/07/Cerba-US-Expansion-Tour-VF.00_04_27_19.Still010.jpg)
Reach out to our flow cytometry team and discover how we can help advance your research
Contact Us